| Registro completo |
| Provedor de dados: |
BJM
|
| País: |
Brazil
|
| Título: |
Carbon sources effect on pectinase production from Aspergillus japonicus 586
|
| Autores: |
Teixeira,Maria F. S.
Lima Filho,José L.
Durán,Nelson
|
| Data: |
2000-10-01
|
| Ano: |
2000
|
| Palavras-chave: |
Pectinases
Aspergillus japonicus
Enzyme
Galacturonase
Pectinesterases
|
| Resumo: |
The effect of different carbon sources on the pectinesterases, endo- and exo-polygalacturonase activities from Aspergillus japonicus 586 was evaluated in liquid media (Manachini solutions) supplemented with different substrate concentrations. The culture medium was inoculated with 5.10(6) spores/ml and mantained under agitation (140 rpm), at 30°C, during 122 h. The enzyme evaluation was carried out 24 h after filtration. The crude extract from A. japonicus 586 indicated that the best enzymatic activities were afforded in the presence of 0.5% pectin (pectinesterease), 0.2% pectin and 0.2% glycerol (endopolygalacturonase), and 0.5% pectin associated to 0.5% glucose (exopolygalacturonase). Carbon sources concentration, isolated or associated, significantly affects the pectinesterase, and endo- and exopolygalacturonase activities. Pectin, glucose and saccharose, when added to the culture medium in high concentrations, exhibited a repression effect on all the analyzed enzymes.
|
| Tipo: |
Info:eu-repo/semantics/article
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400009
|
| Editor: |
Sociedade Brasileira de Microbiologia
|
| Relação: |
10.1590/S1517-83822000000400009
|
| Formato: |
text/html
|
| Fonte: |
Brazilian Journal of Microbiology v.31 n.4 2000
|
| Direitos: |
info:eu-repo/semantics/openAccess
|
